English translation for "transformed gene"
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- 转化基因
Related Translations:
- Example Sentences:
| 1. | On the development and its safety management of the agricultural transforming gene biological products 转基因植物中的标记基因 | | 2. | Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively , we can obtain psc and pac . tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac . , then integrated into puc18 and puc19 , by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70 . for the sake of better screening and examination of transformed gene , we cut plasmid 650 , plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes , then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651 . corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis 分别将从psc 、 pac回收纯化的hsp70正、反义cdna与绒毡层特异表达启动子ta29及nos终止子定向连接,然后插入到puc18 、 puc19中,构建成花药特异表达的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ,分别称作650和651质粒。为了更好地对转化子进行筛选和检测,用hind和ecor分别对650 、 651及3301质粒(含gus报告基因和bar筛选标记基因)进行酶切,将从650和651回收纯化的目的片段与3301质粒进行连接,再对重组子进行平板筛选和酶切分析确定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301质粒中,构建成3301 + 650和3301 + 651表达载体。 |
- Similar Words:
- "transformed clone" English translation, "transformed composite cross section" English translation, "transformed eulerian mean system" English translation, "transformed field equations" English translation, "transformed flow net" English translation, "transformed lattice structure" English translation, "transformed lymphocyte" English translation, "transformed mink fibroblast" English translation, "transformed nucleus" English translation, "transformed phase composition" English translation
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